About WFOM

Wide-Field Optical Mapping (WFOM) is the product of the Columbia University’s Laboratory for Functional Optical Imaging, run by Elizabeth Hillman

../_images/mouse_brain_sq.jpg

Technique

WFOM works by utilizing a high-speed CCD camera’s exposure signal to trigger arrays of high-powered LEDs, set to specified wavelengths.

../_images/wfom_strobe.gif

A paper describing the technique further can be found here.

Applications

WFOM is currently being used in a wide (•͡˘㇁•͡˘) range of applications, such as:

Neurovascular Coupling

../_images/seizure.gif

Seizure caused by tumor growth imaged using WFOM

Resting State Dynamics

../_images/neurovascular.gif

Resting state neural and hemodynamic activity in the awake mouse brain

For a comprehensive list of WFOM applications, visit this page

Why PyWFOM?

PyWFOM combines code previously spread across numerous platforms, devices, and languages into a single, user-friendly Python Package.

Users are able to set camera parameters, stim functions, and data acquisition all in one place.