About WFOM¶

Wide-Field Optical Mapping (WFOM) is the product of the Columbia University’s Laboratory for Functional Optical Imaging, run by Elizabeth Hillman

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Technique¶

WFOM works by utilizing a high-speed CCD camera’s exposure signal to trigger arrays of high-powered LEDs, set to specified wavelengths.

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A paper describing the technique further can be found here.

Applications¶

WFOM is currently being used in a wide (•͡˘㇁•͡˘) range of applications, such as:

Neurovascular Coupling ¶

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Seizure caused by tumor growth imaged using WFOM¶

Resting State Dynamics ¶

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Resting state neural and hemodynamic activity in the awake mouse brain¶

For a comprehensive list of WFOM applications, visit this page

Why PyWFOM?¶

PyWFOM combines code previously spread across numerous platforms, devices, and languages into a single, user-friendly Python Package.

Users are able to set camera parameters, stim functions, and data acquisition all in one place.